(1) Nara Medical University, Kasihara, Japan, (2) Shiga University of Medical Science, Otsu, Japan
We demonstrated that tadalafil improves bladder blood supply and lower urinary tract dysfunction in type 1 diabetic rats (1). In th study, we investigate the effect of tadalafil on diabetic changes bladder proteins to reveal of the mechanisms.
We female Sprague-Dawley rats using a single intraperitoneal injection of streptozotocin. We divided rats into nodiabet (ND), diabet (D), and diabet with tadalafil (DT) groups (n =4 each). The rats were raised for an additional 7 weeks after diabetes. The DT group received oral tadalafil (2 mg/kg/day) for 7 days before . At 7 weeks induction, bladders were resected to measure the change endothelial nitric oxide synthase (eNOS), transforming growth factor-β (TGF-β), collagen type 1, collagen type 3, hypoxia-inducible factor-1α (HIF1α), and vascular endothelial growth factor (VEGF) using Western blotting.
Expression of mature TGF-β and collagen type 1 were significantly lower in the D and DT groups than in the ND group of VEGF was significantly lower in the DT group than in the D group.
Tadalafil improves bladder blood supply, possibly resulting in suppression of angiogenesis in bladder of diabetic rats. However, tadalafil was not effective the impaired productivity of nitric oxide alteration of collagen component.