Attenuation of pelvic nerve activity on bladder underactivity secondary to ischemic bladder rat model induced by bilateral partial iliac arterial occlusion
MD Chu-Tung Lin (1), Professor Chiang-Ting Chien (2), MD, PhD Bing-Juin Chiang (1)
(1) Divisions of Urology, Department of Surgery, Cardinal Tien Hospital, New Taipei, Taiwan , (2) School of life science, National Taiwan Normal University, Taipei, Taiwan
We established a new chronic bladder ischemia model by bilateral partial iliac arterial occlusion (BPAO).
BPAO was done by simultaneous ligation of common iliac arteries and fine needle, then withdrawing the fine needle. Doppler flowmetry and real-time laser speckle contrast imaging were used to quantify the blood flow of common iliac arteries and bladder microcirculation after BPAO on Wistar rats. To determine the voiding dysfunction patterns secondary to BPAO, transcystometrogram was measured We evaluated the oxidative stress by Malondialdehyde (MDA) Assays.
We found 4 weeks BPAO model induced bladder underactivity with decreased voiding amplitude and increased residual urine amount on transcystometrogram. Disruption of phasic contraction and decreased high frequency oscillation (HFO) were found on 4 weeks BPAO group. HFO was significant decreased in duration on BPAO group. The pelvic nerve activity also significantly decreasesd on BPAO group. HFO represents voiding phase mediated by cholinergic signaling.
BPAO significantly increased in MDA. We explored sequential relationship between bladder ischemia related oxidative stress and bladder underactivity.